Theodosiou AM et al."A member of the MAP kinase phosphatase gene family in mouse containing a complex trinucleotide repeat in the coding region".Centromeric polymerase III transcription units in Chironomus pallidivittatus. cdc2MsB, a cognate cdc2 gene from alfalfa, complements the G1/S but not the G2/M transition of budding yeast cdc28 mutants. A Novel Drosophila Minute Locus Encodes Ribosomal Protein S13. Isolation of RNA from Floral Tissue of Rumex acetosa (Sorrel). We present a protocol for small RNA Northern blot analysis that we have successfully used to detect viral miRNAs from cells undergoing lytic infection from. Cloning and expression of a rat brain L-glutamate transporter. Direct mRNA Isolation Using Magnetic Oligo (dT) Beads: A Protocol for All Types of Cell Cultures, Animal and Plant Tissues. This is particularly important when working with small and precious samples or when isolating low-abundance transcripts. Using a solid-phase enables all procedures to be conducted in a single tube and helps ensure that losses caused by multiple sample manipulations are minimized. The unique paramagnetic properties of Dynabeads facilitate easy and efficient handling, whilst also eliminating the requirement for centrifugation, precipitation, and the use of hazardous chemicals. The flexible and scalable method fits any sample size, and is particularly useful for northern analysis starting from small amounts of sample material. Dynabeads Oligo(dT)25 can be regenerated and reused at least four times.ĭynabeads Oligo(dT)25 allows the isolation of mRNA directly from crude samples or from total RNA.Stringent hybridization and washing buffers along with strong RNase inhibition helps ensure isolation of full-length, intact mRNA.Easy and efficient magnetic handling minimizes losses caused by sample manipulations.The isolation protocol can be scaled up or down.Isolate mRNA from total RNA or directly from crude samples in only 15 minutes.Benefits of mRNA isolation with Dynabeads Oligo(dT)25
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